Click image to see more details
Product Info Summary
| SKU: | M00067-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Insulin Rabbit Monoclonal Antibody
SKU/Catalog Number
M00067-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Insulin Rabbit Monoclonal Antibody catalog # M00067-1. Tested in IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Insulin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00067-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DEA-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Insulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00067-1 is reactive to INS in Human, Mouse, Rat
Applications
M00067-1 is guaranteed for IF, IHC, ICC Boster Guarantee
Observed Molecular Weight
25 kDa
Calculated molecular weight
11.981kDa
Background of INS
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:500-1:1000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded mouse pancreas, using Insulin Antibody.
Click image to see more details
Immunofluorescent analysis of BxPC-3 cells, using Insulin Antibody .
Protein Target Info & Infographic
Gene/Protein Information For INS (Source: Uniprot.org, NCBI)
Gene Name
INS
Full Name
Insulin
Weight
11.981kDa
Superfamily
insulin family
Alternative Names
IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin INS IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4 insulin insulin|preproinsulin|proinsulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INS, check out the INS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Insulin Rabbit Monoclonal Antibody (M00067-1)
Hello CJ!
M00067-1 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dong Yan,Pengcheng Fan,Wenlong Sun,Qianzhi Ding,Wei Zheng,Weidi Xiao,Bowei Zhang,Tao Zhang,Tao Zhang,Jiahui Shi,Xiaojuan Chen,Peiru Chen,Jie Zhang,Ying Hao,Xinguang Sun,Xu Pang,Yuesheng Dong,Ping Xu,Liyan Yu,Baiping Ma,Anemarrhena asphodeloides modulates
Species: Rat,A. asphodeloides
M00067-1 usage in article: APP:IF, SAMPLE:PANCREAS TISSUE, DILUTION:1:100
Huo K,Li X,Hu W,Song X,Zhang D,Zhang X,Chen X,Yuan J,Zuo J,Wang X.RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis.Front Endocrinol (Lausanne). 2020 Apr 9;11:194.doi:10.3389/fendo.2020.00194.PMID
Species: Rat
M00067-1 usage in article: APP:IHC, SAMPLE:PANCREATIC TISSUE, DILUTION:1:100
Gynura divaricata rich in 3, 5%u2212/4, 5-dicaffeoylquinic acid and chlorogenic acid reduces islet cell apoptosis and improves pancreatic function in type 2 diabetic %u2026
RIG-I inhibits pancreatic ? cell proliferation through competitive binding of activated Src
Interference effect of oral administration of mulberry branch bark powder on the incidence of type II diabetes in mice induced by streptozotocin
miR-487b mitigates chronic heart failure through inhibition of the IL-33/ST2 signaling pathway
Liu D, Zhou Y, Peng Y, Su P, Li Z, Xu Q, Tu Y, Tian X, Yang H, Wu Z, Mei W, Gao F. Front Mol Neurosci. 2018 Mar 6;11:72. doi: 10.3389/fnmol.2018.00072. eCollection 2018. Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of...
Lu J, Gu Yp, Xu X, Liu Ml, Xie P, Song Hp. World J Gastroenterol. 2005 Jun 14;11(22):3426-30. Adult Islets Cultured In Collagen Gel Transdifferentiate Into Duct-Like Cells.
Hu N, Yuan L, Li Hj, Huang C, Mao Qm, Zhang Yy, Lin M, Sun Yq, Zhong Xy, Tang P, Lu X. Evid Based Complement Alternat Med. 2013;2013:180721. Doi: 10.1155/2013/180721. Epub 2013 May 29. Anti-Diabetic Activities Of Jiaotaiwan In Db/Db Mice By Augmen...
Ding H, Zhu T, Yin X, Liu J, Zhang L, Bernier M, Zhao R. Acta Biochim Biophys Sin (Shanghai). 2014 Jul;46(7):582-9. Doi: 10.1093/Abbs/Gmu034. Epub 2014 May 14. Pyrrolidine Dithiocarbamate Protects Pancreatic ??-Cells From Oxidative Damage Through ...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Insulin Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Insulin Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
2 Customer Q&As for Anti-Insulin Rabbit Monoclonal Antibody
Question
Can we use basic antigen retrieval with M00067-1 antibody, as alternatives for citrate buffer?
Verified customer
Asked: 2019-10-24
Answer
For the Anti-Insulin Rabbit Monoclonal Antibody M00067-1, it is suggested to use Tris-EDTA buffer for the antigen retrieval buffer with the compositions; 10 mM Tris(hydroxymethyl)methyl aminomethane THAM, 1 mM EDTA, 0.05% Tween 20, pH 9.0.
Boster Scientific Support
Answered: 2019-10-29
Question
Would you please check the IHC image for this mAb: https://www.bosterbio.com/anti-insulin-rabbit-monoclonal-antibody-m00067-1-boster.html?options=cart I
Verified Customer
Verified customer
Asked: 2014-06-11
Answer
We have updated the image. https://www.bosterbio.com/anti-insulin-rabbit-monoclonal-antibody-m00067-1-boster.html?options=cart
Boster Scientific Support
Answered: 2014-06-11
